Trehalose is not a universal solution for solid lipid nanoparticles freeze-drying

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Doktorovova, S (Author) Other; Shegokar, R (Author) Other; Fernandes, L (Author) Other; Martins Lopes, P (Author) Other; Silva, AM (Author) Other; Mueller, RH (Author) Other; Souto, EB (Author) Other

Journal

Title: Pharmaceutical Development and Technology

Vol. 19

Pages: 922-929

ISSN: 1083-7450

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 25 Citations

Other information

Authenticus ID: P-006-HT0

DOI: 10.3109/10837450.2013.840846

Abstract (EN): Objective: To prepare stable and easy to handle formulation of solid lipid nanoparticles (SLNs) by freeze-drying with or without cryoprotectants, as appropriate. Materials and methods: SLNs were freeze-dried without cryoprotectants or with cryoprotectants in quantities selected by freeze-thaw test (sucrose, glucose) or literature search (trehalose, maltose). Appearance, re-dispersability and size distribution of re-dispersed samples were evaluated. Results: SLN could be freeze-dried using 10% sucrose, trehalose or maltose. Trehalose was effective in protecting one of presented formulations that was already very stable on its own; its efficiency in protecting other two formulations was limited. Discussion: Our results are in line with various reports of successful freeze-drying of SLN, but considering the stability of original dispersions, no improvement was achieved. Conclusion: We confirmed that trehalose is among the most suitable cryoprotectant for SLN, however it did not improve shelf-life of the most stable formulation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8