Urinary Cysteinyl Leukotrienes as Biomarkers of Endothelial Activation, Inflammation and Oxidative Stress and Their Relationship with Organ Dysfunction in Human Septic Shock

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Reina-Couto, M (Author) Other; Santos-Oliveira, M (Author) Other; Pereira-Terra, P (Author) Other; Silva-Pereira, C (Author) Other; Quelhas-Santos, J (Author) FMUP; Duarte, A (Author) Other; Martins, S (Author) Other; Serrao, P (Author) Other; Dias C (Author) FMUP; Morato, M (Author) FFUP; guimaraes, jt (Author) FMUP; Roncon-Albuquerque R Jr (Author) FMUP; Paiva, J-A (Author) FMUP; Albino-Teixeira, A (Author) Other; Sousa, T (Author) FMUP

Journal

Title: Biomedicines

Vol. 10

Final page: 2845

Publisher: MDPI

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 2 Citations

Other information

Authenticus ID: P-00X-ENE

DOI: 10.3390/biomedicines10112845

Abstract (EN): Cysteinyl leukotrienes (CysLT) are potent vascular leakage-promoting agents but have been scarcely explored in human septic shock (SS). We evaluated CysLT at admission and during hospitalization and their correlation with endothelial dysfunction, inflammation, oxidative stress, the renin-angiotensin-aldosterone system, and cardiac, renal, respiratory, and hepatic parameters in SS patients. Blood and spot-urine samples were collected at days 1-2 (admission), 3-4, and 5-8 in SS patients (n = 13) and at a single time point in controls (n = 22). Urinary CysLT (u-CysLT) and isoprostanes, plasma, and urinary angiotensinogen, serum myeloperoxidase, and IL-10 were quantified by ELISA. Serum intercellular-adhesion molecule-1, vascular cell-adhesion molecule-1, E-selectin, tumor necrosis factor-alpha, IL-1 beta, and IL-6 were measured by multiplex immunoassays. Routine markers were evaluated using automated analyzers. At admission, SS patients had increased u-CysLT, endothelial activation, inflammation, oxidative stress, and plasma and urinary angiotensinogen, as well as cardiac, respiratory, hepatic, and renal injury/dysfunction. There were no changes in u-CysLT during hospitalization. Both correlation and multivariate analyses showed positive relationships of u-CysLT with endothelial activation, inflammation, oxidative stress, proteinuria, and hepatic injury/dysfunction markers. These results suggest that u-CysLT may be potential non-invasive biomarkers for monitoring the pathophysiological mechanisms underlying SS, as well as putative therapeutic targets.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 26