Coimmobilization of L-asparaginase and glutamate dehydrogenases onto highly activated supports

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Balcao, VM (Author) Other; Mateo, C (Author) Other; Fernandez Lafuente, R (Author) Other; F. Xavier Malcata (Author) Other; Guisan, JM (Author) Other

Journal

Title: Enzyme and Microbial Technology

Vol. 28

Pages: 696-704

ISSN: 0141-0229

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 32 Citations

Scopus - 33 Citations

Other information

Authenticus ID: P-000-VHS

DOI: 10.1016/s0141-0229(01)00307-6

Abstract (EN): In the present research work, production of coimmobilized derivatives of L-asparaginase and glutamate dehydrogenase was attempted. Comparison of immobilization of each enzyme independently with coimmobilization of the two enzymes unfolded important advantages of the Latter, namely a decrease in the induction period (time before the maximum reaction rate is virtually achieved! and an increase in the maximum reaction rate. The effectiveness of the independent enzyme derivatives was low; however, it was enhanced by three-fold when the enzymes were coimmobilized onto the same agarose-glutaraldehyde support. Each supporting agarose bead may in fact be viewed as a nano-reactor with in situ reaction and separation (i.e. elimination of the ammonia formed), with the nanoenvironment surrounding each enzyme molecule being essentially devoid of steric hindrance.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9