Fast and reliable ICP-MS quantification of palladium and platinum-based drugs in animal pharmacokinetic and biodistribution studies

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Vojtek, M (Author) Other; Pinto, E (Author) Other; Goncalves Monteiro, S (Author) Other; Agostinho Almeida (Author) FFUP; Marques, MPM (Author) Other; Mota Filipe, H (Author) Other; Ferreira, IMPLVO (Author) Other; Diniz, C (Author) FFUP

Journal

Title: Analytical Methods

Vol. 12

Pages: 4806-4812

ISSN: 1759-9660

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-00S-R93

DOI: 10.1039/d0ay01328e

Abstract (EN): Palladium-(Pd)-based drugs are emerging as alternatives to platinum (Pt) anticancer chemotherapeutics, which increases the need for efficient and suitable procedures of Pd analysis in reduced amounts of pre-clinical animal samples. Herein, an ICP-MS (inductively coupled plasma-mass spectrometry) method was developed and validated for simple and fast analysis of Pd/Pt-based drugs in 11 distinct biological matrices (adipose tissue, muscle, liver, kidney, spleen, testis, heart, lungs, brain, blood and serum). The critical variables affecting sample preparation and Pd/Pt extraction were optimized using two-level (2(k)) factorial and central composite designs. Biological samples (50 mg) were digested in closed tubes with a screw cap, using a 3 : 1 (v/v) mixture of nitric acid (900 mu L) and hydrochloric acid (300 mu L) for 60 min in a 90 degrees C water bath. Full method validation using in-house materials showed a LOD of 0.001 mu g L-1, linear dynamic range from 0.025-10 mu g L-1(R-2= 0.9999 for Pd;R-2= 0.9998 for Pt), good repeatability (CV: 0.02-1.9%) and intermediate precision (CV: 0.52-1.53%) for both the studied metals. The accuracy ranged from 83.5-105.1% considering microwave-assisted digestion as the reference method. The developed and validated method allows the processing of hundreds of biological samples simultaneously, with low reagent and sample consumption. Therefore, the method is highly suitable for analysis of novel Pd/Pt-based drugs in pharmaco-toxicokinetic and biodistribution animal studies that involve a large number of multi-organ samples.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7