Methotrexate enhances 3T3-L1 adipocytes hypertrophy

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Claudia Marques (Author) Other; Diana Teixeira (Author) Other; Ana Cunha (Author) Other; Manuela Meireles (Author) FMUP; Diogo Pestana (Author) Other; Elisa Keating (Author) FMUP; Conceicao Calhau (Author) FMUP; Rosario Monteiro (Author) FMUP; Ana Faria (Author) FCUP

Journal

Title: Cell Biology and Toxicology

Vol. 29 No. 4

Pages: 293-302

ISSN: 0742-2091

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 6 Citations

ISI Web of Science

Scopus - 6 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Basic medicine

CORDIS: Health sciences

Other information

Authenticus ID: P-006-8GT

DOI: 10.1007/s10565-013-9255-0

Abstract (EN): Methotrexate (MTX) is broadly used in the treatment of chronic inflammatory diseases such as rheumatoid arthritis (RA). The prevalence of metabolic syndrome (MeS) in patients with this condition is relatively high. Given the importance of adipose tissue in the development of obesity metabolic complications, this study aimed to investigate the effect of methotrexate on preadipocyte proliferation, adipogenesis, and glucose uptake by adipocytes. 3T3-L1 preadipocytes proliferation was evaluated by sulforhodamine B staining and H-3-thymidine incorporation, after 24 or 48 h of treatment with MTX (0.1 and 10 mu M). Preadipocytes were induced to differentiate with an appropriate adipogenic cocktail in the presence or absence of MTX. Adipogenesis was determined by measuring lipid accumulation after staining with oil red O. H-3-Deoxyglucose (H-3-DG) uptake was determined by liquid scintillation counting. MTX treatment reduced culture protein content in a concentration-dependent manner and H-3-thymidine incorporation (P < 0.05). MTX (0.1 mu M) treatment increased lipid accumulation and basal H-3-DG uptake by adipocytes (P < 0.05). In 0.1 mu M MTX-treated adipocytes, insulin stimulation did not result in an increase of H-3-DG uptake, contrarily to what was observed in control cells. These results demonstrate that methotrexate interferes with adipocyte proliferation and promotes the hypertrophic growth of adipocytes. These molecular effects may have implications on metabolic profile of RA patients treated with MTX.

Language: English

Type (Professor's evaluation): Scientific

Notes: <a href="http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord&KeyUT=:000323510600008">Indexado na ISI Web of Science</a>

No. of pages: 10