The best CRISPR/Cas9 versus RNA interference approaches for Arabinogalactan proteins' study

• Type: Another Publication in an International Scientific Journal
• Date: 2020-01-08

Authors

Moreira, D (Author) Other; Coimbra, S (Author) FCUP; Lopes, AL (Author) FCUP

Journal

Title: Molecular Biology Reports

Vol. 47

Pages: 2315-2325

ISSN: 0301-4851

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-00R-MBY

DOI: 10.1007/s11033-020-05258-0

Abstract (EN): Arabinogalactan Proteins (AGPs) are hydroxyproline-rich proteins containing a high proportion of carbohydrates, widely spread in the plant kingdom. AGPs have been suggested to play important roles in plant development processes, especially in sexual plant reproduction. Nevertheless, the functions of a large number of these molecules, remains to be discovered. In this review, we discuss two revolutionary genetic techniques that are able to decode the roles of these glycoproteins in an easy and efficient way. The RNA interference is a frequently technique used in plant biology that promotes genes silencing. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 9 (CRISPR/Cas9), emerged a few years ago as a revolutionary genome-editing technique that has allowed null mutants to be obtained in a wide variety of organisms, including plants. The two techniques have some differences between them and depending on the research objective, these may work as advantage or disadvantage. In the present work, we propose the use of the two techniques to obtain AGP mutants easily and quickly, helping to unravel the role of AGPs, surely a great asset for the future.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11