Multi-Organ NMR Metabolomics to Assess In Vivo Overall Metabolic Impact of Cisplatin in Mice

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Carneiro, TJ (Author) Other; Araujo, R (Author) Other; Vojtek, M (Author) Other; Goncalves Monteiro, S (Author) Other; Diniz, C (Author) FFUP; de Carvalho, ALMB (Author) Other; Marques, MPM (Author) Other; Gil, AM (Author) Other

Journal

Title: Metabolites

Vol. 9

Final page: 279

ISSN: 2218-1989

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00R-BZ8

DOI: 10.3390/metabo9110279

Abstract (EN): This work describes, to our knowledge, the first NMR metabolomics analysis of mice kidney, liver, and breast tissue in response to cisplatin exposure, in search of early metabolic signatures of cisplatin biotoxicity. Balb/c mice were exposed to a single 3.5 mg/kg dose of cisplatin and then euthanized; organs (kidney, liver, breast tissue) were collected at 1, 12, and 48 h. Polar tissue extracts were analyzed by NMR spectroscopy, and the resulting spectra were studied by multivariate and univariate analyses. The results enabled the identification of the most significant deviant metabolite levels at each time point, and for each tissue type, and showed that the largest metabolic impact occurs for kidney, as early as 1 h post-injection. Kidney tissue showed a marked depletion in several amino acids, comprised in an overall 13-metabolites signature. The highest number of changes in all tissues was noted at 12 h, although many of those recovered to control levels at 48 h, with the exception of some persistently deviant tissue-specific metabolites, thus enabling the identification of relatively longer-term effects of cDDP. This work reports, for the first time, early (1-48 h) concomitant effects of cDDP in kidney, liver, and breast tissue metabolism, thus contributing to the understanding of multi-organ cDDP biotoxicity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17