Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from wild animals

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Carla Dias (Author) FEUP; Anabela Borges (Author) FEUP; Diana Oliveira (Author) FEUP; Antonio Martinez-Murcia (Author) Other; Maria José Saavedra (Author) Other; Manuel Simões (Author) FEUP

Journal

Title: PeerJ

Vol. 6 No. e4974

Pages: 1-20

ISSN: 2167-8359

Publisher: PeerJ

Indexing

ISI Web of Knowledge - 2 Citations

ISI Web of Science

Scopus - 22 Citations

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00N-ZFW

DOI: 10.7717/peerj.4974

Abstract (EN): Background. The "One Health" concept recognizes that human health and animal health are interdependent and bound to the health of the ecosystem in which they (co)exist. This interconnection favors the transmission of bacteria and other infectious agents as well as the flow of genetic elements containing antibiotic resistance genes. This problem is worsened when pathogenic bacteria have the ability to establish as biofilms. Therefore, it is important to understand the characteristics and behaviour of microorganisms in both planktonic and biofilms states from the most diverse environmental niches to mitigate the emergence and dissemination of resistance. Methods. The purpose of this work was to assess the antibiotic susceptibility of four bacteria (Acinetobacter spp., Klebsiella pneumoniae, Pseudomonas fluorescens and Shewanella putrefaciens) isolated from wild animals and their ability to form biofilms. The effect of two antibiotics, imipenem (IPM) and ciprofloxacin (CIP), on biofilm removal was also assessed. Screening of resistance genetic determinants was performed by PCR. Biofilm tests were performed by a modified microtiter plate method. Bacterial surface hydrophobicity was determined by sessile drop contact angles. Results. The susceptibility profile classified the bacteria as multidrug-resistant. Three genes coding for beta-lactamases were detected in K. pneumoniae (TEM, SHV, OXA-aer) and one in P. fluorescens (OXA-aer). K. pneumoniae was the microorganism that carried more beta-lactamase genes and it was the most proficient biofilm producer, while P. fluorescens demonstrated the highest adhesion ability. Antibiotics at their MIC, 5 x MIC and 10 x MIC were ineffective in total biofilm removal. The highest biomass reductions were found with IPM (54% at 10 x MIC) against K. pneumoniae biofilms and with CIP (40% at 10 x MIC) against P. fluorescens biofilms. Discussion. The results highlight wildlife as important host reservoirs and vectors for the spread of multidrug-resistant bacteria and genetic determinants of resistance. The ability of these bacteria to form biofilms should increase their persistence.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 20