Treatment of Francisella infections via PLGA- and lipid-based nanoparticle delivery of antibiotics in a zebrafish model

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Ulanova, LS (Author) Other; Pinheiro, M (Author) Other; Vibe, C (Author) Other; Nunes, C (Author) Other; Misaghian, D (Author) Other; Wilson, S (Author) Other; Zhu, K (Author) Other; Fenaroli, F (Author) Other; Winther Larsen, HC (Author) Other; Salette Reis (Author) FFUP; Griffiths, G (Author) Other

Journal

Title: Diseases of Aquatic Organisms

Vol. 125

Pages: 19-29

ISSN: 0177-5103

Publisher: Inter-Research Science Publishing

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00N-G7F

DOI: 10.3354/dao03129

Abstract (EN): We tested the efficiency of 2 different antibiotics, rifampicin and oxolinic acid, against an established infection caused by fish pathogen Francisella noatunensis ssp. orientalis (F.n.o.) in zebrafish. The drugs were tested in the free form as well as encapsulated into bio degradable nanoparticles, either polylactic-co-glycolic acid (PLGA) nanoparticles or nano structured lipid carriers. The most promising therapies were PLGA-rifampicin nanoparticles and free oxolinic acid; the PLGA nanoparticles significantly delayed embryo mortality while free oxolinic acid prevented it. Encapsulation of rifampicin in both PLGA and nanostructured lipid carriers enhanced its efficiency against F.n.o. infection relative to the free drug. We propose that the zebrafish model is a robust, rapid system for initial testing of different treatments of bacterial diseases important for aquaculture.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11