An improved leaping detector for flow analysis applied to iron speciation in drugs

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Santos, SRB (Author) Other; Araujo, MCU (Author) Other; Honorato, RS (Author) Other; Zagatto, EAG (Author) Other; Lima, JLFC (Author) FFUP; Lapa, RAS (Author) FFUP

Journal

Title: Journal of Automated Methods and Management in Chemistry

Vol. 22

Pages: 83-88

ISSN: 1463-9246

Publisher: HINDAWI PUBLISHING CORPORATION

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 1 Citation

Other information

Authenticus ID: P-005-69J

DOI: 10.1155/s1463924600000092

Abstract (EN): A low inner volume (ca. 64 ml) probe was built up in an injector-commutator in order to behave as a photometric leaping detector in flow analysis. It comprises a bicolour light-emitting diode (BLED), as a source of pulsed radiation in the red and green visible region, and two phototransistors as transducers. Sample injection, detector relocation, analytical signal recording, data treatment and definition of the spectral working range were computer-controlled. The feasibility of the system was initially demonstrated in the flow-injection speciation of iron, and the overall standard deviation of results was estimated as +/-1.6 and +/-1.4% for 1.6-4.0 mg l(-1) Fe(II) or total iron after eightfold processing of synthetic samples. The system was further applied to drug analysis: the mean deviations of results for typical samples mere estimated as +/-5.2 and +/-3.3%, and the relative standard deviation as +/-1.6 and +/-1.3% for Fe(II) and total iron, respectively. Results were compared with those obtained by a conventional spectrophotometric procedure and no statistic differences at the 95% confidence level mere found. In relation to an earlier system with multi-site detection, the proposed system is more stable, presenting low drift with a relative standard deviation of 0.026% and 0.039% for measurements (n = 120 during 4 h of observation) with green and red emission. It is also faster with a sampling rate of 133 h(-1) and carryover problems are not found. The possibility of compensating the Schlieren noise by dual-wavelength spectrophotometry is discussed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6