MESENCHYMAL STEM CELL RECRUITMENT BY STROMAL DERIVED FACTOR-1-DELIVERY SYSTEMS BASED ON CHITOSAN/POLY(gamma-GLUTAMIC ACID) POLYELECTROLYTE COMPLEXES

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Goncalves, RM (Author) Other; Antunes, JC (Author) Other; Mário Adolfo Barbosa (Author) FEUP

Journal

Title: European Cells and Materials

Vol. 23

Pages: 249-261

ISSN: 1473-2262

Publisher: AO Research Institute Davos

Indexing

ISI Web of Knowledge - 19 Citations

Scopus - 17 Citations

Other information

Authenticus ID: P-002-FPH

Abstract (EN): Human mesenchymal stem cells (hMSCs) have an enormous potential for tissue engineering and cell-based therapies. With a potential of differentiation into multiple lineages and immune-suppression, these cells play a key role in tissue remodelling and regeneration. Here a method of hMSC recruitment is described, based on the incorporation of a chemokine in Chitosan (Ch)/Poly(gamma-glutamic acid) (gamma-PGA) complexes. Ch is a non-oxic, cationic polysaccharide widely investigated. gamma-PGA is a hydrophilic, non-toxic, biodegradable and negatively charged poly-amino acid. Ch and gamma-PGA, being oppositely charged, can be combined through electrostatic interactions. These biocompatible structures can be used as carriers for active substances and can be easily modulated in order to control the delivery of drugs, proteins, DNA, etc. Using the layer-by-layer method, Ch and gamma-PGA were assembled into polyelectrolyte multilayers films (PEMs) with thickness of 120 nm. The chemokine stromal-derived factor-1 (SDF-1) was incorporated in these complexes and was continuously released during 120 h. The method of SDF-1 incorporation is of crucial importance for polymers assembly into PEMs and for the release kinetics of this chemokine. The Ch/gamma-PGA PEMs with SDF-1 were able to recruit hMSCs, increasing the cell migration up to 6 fold to a maximum of 16.2 +/- 4.9 cells/mm(2). The controlled release of SDF-1 would be of great therapeutic value in the process of hMSC homing to injured tissues. This is the first study suggesting Ch/gamma-PGA PEMs as SDF-1 reservoirs to recruit hMSCs, describing an efficient method of chemokine incorporation that allows a sustained released up to 5 days and that can be easily scaled-up.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13