Effect of Myricetin, Pyrogallol, and Phloroglucinol on Yeast Resistance to Oxidative Stress

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Vanda Mendes (Author) ICBAS; Rita Vilaça (Author) ICBAS; Victor Freitas (Author) FCUP; Pedro Gaspar Ferreira (Author) ICBAS; Nuno Mateus (Author) FCUP; Vitor Costa (Author) ICBAS

Journal

Title: Oxidative Medicine and Cellular Longevity

Vol. 2015 No. Article ID 782504, 10 pages

Pages: 1-10

ISSN: 1942-0900

Publisher: Hindawi

Indexing

ISI Web of Knowledge - 40 Citations

Scopus - 43 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-00G-4TJ

DOI: 10.1155/2015/782504

Abstract (EN): The health beneficial effects of dietary polyphenols have been attributed to their intrinsic antioxidant activity, which depends on the structure of the compound and number of hydroxyl groups. In this study, the protective effects of pyrogallol, phloroglucinol, and myricetin on the yeast Saccharomyces cerevisiae were investigated. Pyrogallol and myricetin, which have a pyrogallol structure in the B ring, increased H2O2 resistance associated with a reduction in intracellular oxidation and protein carbonylation, whereas phloroglucinol did not exert protective effects. The acquisition of oxidative stress resistance in cells pretreated with pyrogallol and myricetin was not associated with an induction of endogenous antioxidant defences as assessed by the analysis of superoxide dismutase and catalase activities. However, myricetin, which provided greater stress resistance, prevented H2O2 - induced glutathione oxidation. Moreover, myricetin increased the chronological lifespan of yeast lacking the mitochondrial superoxide dismutase (Sod2p), which exhibited a premature aging phenotype and oxidative stress sensitivity. These findings show that the presence of hydroxyl groups in the ortho position of the B ring in pyrogallol and myricetin contributes to the antioxidant protection afforded by these compounds. In addition, myricetin may alleviate aging-induced oxidative stress, particularly when redox homeostasis is compromised due to downregulation of endogenous defences present in mitochondria.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10