Toward a genome-wide approach for detecting hybrids: informative SNPs to detect introgression between domestic cats and European wildcats (Felis silvestris)

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Oliveira, R (Author) Other; Randi, E (Author) Other; Mattucci, F (Author) Other; Kurushima, JD (Author) Other; Lyons, LA (Author) Other; Alves, PC (Author) FCUP

Journal

Title: Heredity

Vol. 115

Pages: 195-205

ISSN: 0018-067X

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 44 Citations

Scopus - 45 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-00G-G2Z

DOI: 10.1038/hdy.2015.25

Abstract (EN): Endemic gene pools have been severely endangered by human-mediated hybridization, which is posing new challenges in the conservation of several vertebrate species. The endangered European wildcat is an example of this problem, as several natural populations are suffering introgression of genes from the domestic cat. The implementation of molecular methods for detecting hybridization is crucial for supporting appropriate conservation programs on the wildcat. In this study, genetic variation at 158 single-nucleotide polymorphisms (SNPs) was analyzed in 139 domestic cats, 130 putative European wildcats and 5 captive-bred hybrids (N = 274). These SNPs were variable both in wild (H-E = 0.107) and domestic cats (H-E = 0.340). Although we did not find any SNP that was private in any population, 22 SNPs were monomorphic in wildcats and pairwise F-CT values revealed marked differences between domestic and wildcats, with the most divergent 35 loci providing an average F-CT>0.74. The power of all the loci to accurately identify admixture events and discriminate the different hybrid categories was evaluated. Results from simulated and real genotypes show that the 158 SNPs provide successful estimates of admixture, with 100% hybrid individuals (two to three generations in the past) being correctly identified in STRUCTURE and over 92% using the NEWHYBRIDS' algorithm. None of the unclassified cats were wrongly allocated to another hybrid class. Thirty-five SNPs, showing the highest FCT values, provided the most parsimonious panel for robust inferences of parental and first generations of admixed ancestries. This approach may be used to further reconstruct the evolution of wildcat populations and, hopefully, to develop sound conservation guidelines for its legal protection in Europe.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11