Inhibitory activity of polyhydroxycarboxylate chelators against recombinant NF-kappa B p50 protein-DNA binding

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Sharma, RK (Author) Other; Pande, V (Author) Other; Ramos, MJ (Author) FCUP; Rajor, HK (Author) Other; Chopra, S (Author) Other; Meguro, K (Author) Other; Inoue, J (Author) Other; Otsuka, M (Author) Other

Journal

Title: Bioorganic Chemistry

Vol. 33

Pages: 67-81

ISSN: 0045-2068

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 11 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-3XR

DOI: 10.1016/j.bioorg.2004.12.001

Abstract (EN): The inhibitory effect of 7,8-dihydroxy-4-methylcoumarin (7,8-DHMC), 5,7-dihydroxy-4-methylcoumarin (5,7-DHMC), and gallic acid on the DNA binding of recombinant p50 protein and their interaction with zinc ion were studied. Electrophoretic mobility shift assay (EMSA) using p50 and biotin labeled DNA has shown that gallic acid is more effective than the dihydroxycoumarins in inhibiting the p50-DNA binding. Molecular modeling studies suggest an explanation for these observations. Effect of the addition of zinc after p50-DNA-binding inhibition by gallic acid was also studied. Chemical speciation and formation constant studies show that gallic acid forms a more stable 1: 1 complex with zinc ion in comparison to the dihydroxycoumarins.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15