The human autoantigen MCP1 is required during early stages of DNA replication

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Bronze da Rocha, E (Author) FFUP; Novoa, A (Author) Other; Cunha, C (Author) Other; do Carmo Fonseca, M (Author) Other; Staines, NA (Author) Other; Sunkel, CE (Author) ICBAS

Journal

Title: Chromosome Research

Vol. 8

Pages: 699-711

ISSN: 0967-3849

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-195

DOI: 10.1023/a:1012097704355

Abstract (EN): Metaphase chromosome protein 1 (MCP1) is a nuclear autoantigen that is associated with condensed chromosomes throughout mitosis. During interphase, this antigen shows a speckle distribution in the nucleus, excluding the nucleolus. Additionally, MCP1 binds tightly to the scaffold/matrix component of nuclei and isolated chromosomes. In order to determine the in-vivo localization of the antigen, we have expressed MCP1 fused to EGFP in tissue culture cells. The results demonstrate that MCP1 is located in the nucleus during interphase and during mitosis associates tightly to condensed chromosomes. Furthermore, microinjection of specific antibody confirms these results. We have used a specific monoclonal antibody (mAb 402) against MCP1 to assess the function of this antigen during cell cycle progression. HeLa and Ptk-2 cells that were microinjected into the nucleus and/or cytoplasm at G1/S and very early S phase were not able to progress and complete DNA replication. However, injection of mAb 402 at mid or late S phase does not prevent completion of DNA replication and subsequent progression into mitosis. Microinjection of mAb 402 in Ptk-2 cells synchronized in mitosis did not interfere with progression of mitosis and cells divided. Our results suggest that MCP1 is required at the G1/S transition and during early S phase.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13