Ultrasonication of insulin-loaded microgel particles produced by internal gelation: Impact on particle's size and insulin bioactivity

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Santos, AC (Author) Other; Cunha, J (Author) Other; Veiga, F (Author) Other; Cordeiro Da Silva, A (Author) FFUP; Ribeiro, AJ (Author) Other

Journal

Title: Carbohydrate Polymers

Vol. 98

Pages: 1397-1408

ISSN: 0144-8617

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 18 Citations

Scopus - 22 Citations

Other information

Authenticus ID: P-008-EF9

DOI: 10.1016/j.carbpol.2013.06.063

Abstract (EN): Alginate-dextran sulfate (ADS) microgel has been used to protect insulin from gastrointestinal attack and as a carrier to promote insulin permeation through intestinal epithelium. The throughput of ADS submicron particles generation by emulsification/internal gelation is limited by its wide size distribution. The aim of this work was to study the recovery protocol influence on ADS particles through the determination of its impact on particles' size distribution and bioactivity. ADS particles showed a wide and multimodal distribution, characterized by a high aggregation phenomenon. In an attempt to reverse particles' tendency to aggregate and to homogenize particle size ADS populations were submitted to ultrasonication, while particle size distribution, physical and chemical stability, and the bioactivity of entrapped insulin were investigated. After ultrasonication a narrower particle population shifted to the nanoscale, with higher physical stability and significant insulin bioactivity was obtained. Emulsification internal/gelation followed by ultrasonication constituted a valid strategy to obtain ADS particles at the submicron range, with high stability and without significantly compromising insulin bioactivity, so offering promises, under previously well established conditions, to evaluate impact of ADS particle's size on biopharmaceutical and pharmacokinetics phases.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12