HPLC/UV analysis of proteins in dairy products using a hydrophobic interaction chromatographic column

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Ferreira, IMPLVO (Author) FFUP; Mendes, E (Author) Other; Ferreira, MA (Author) Other

Journal

Title: Analytical Sciences

Vol. 17

Pages: 499-501

ISSN: 0910-6340

Publisher: Japan Society for Analytical Chemistry

Indexing

ISI Web of Knowledge - 38 Citations

Scopus - 33 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-VWA

DOI: 10.2116/analsci.17.499

Abstract (EN): High-performance liquid chromatography using a Chrompack P-300-RP column containing a polystyrene-divinylbenzene copolymer-based packing was examined to analyze bovine milk protein components. The separation of major raw-milk proteins could be performed rapidly and reliably with this HPLC/UV method. The determinations were performed in the linear ranges of 0.01-2.0 mg/ml for alpha -lactalbumin, 0.04-2.5 mg/ml for caseins and 0.02-2.0 mg/ml for beta -lactoglobulin. The validity of the method was verified. Since the chromatographic column enabled the quantification of only "native" milk proteins, the extent of denaturation and loss of milk proteins could be examined. Thus, evaluation of heat-induced proteins denaturation was carried out in raw milk heated for 5 min at pre-determined temperatures.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3