Immobilization of commercial laccase onto green coconut fiber by adsorption and its application for reactive textile dyes degradation

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Raquel O. Cristóvão (Author) FEUP; Ana P. M. Tavares (Author) FEUP; Ana Iraidy Brígida (Author) Other; José M. Loureiro (Author) FEUP; Rui A. R. Boaventura (Author) FEUP; Eugénia A. Macedo (Author) FEUP; Maria Alice Z. Coelho (Author) Other

Journal

Title: Journal of Molecular Catalysis B: Enzymatic

Vol. 72 No. 1-2

Pages: 6-12

ISSN: 1381-1177

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 120 Citations

ISI Web of Science

Scopus - 136 Citations

Scientific classification

FOS: Engineering and technology > Other engineering and technologies

CORDIS: Technological sciences

Associated Projects

LA 20 - Projeto Estratégico-LA 20 - 2011-2012

4.1.3-2008/CAPES/CPLP - Enzymatic Catalysis for Degradation of Dyes/Phenolic Compounds from Industrial Effluents

Other information

Authenticus ID: P-002-MDF

DOI: 10.1016/j.molcatb.2011.04.014

Abstract (EN): An effort has been made to find a cheaper, easily available and simple alternative for the immobilization of enzymes and subsequent utilization at large scale in textile wastewater treatment. Commercial laccase was immobilized for the first time on an agroindustrial residue, green coconut fiber, by physical adsorption. The effect of the immobilization conditions (enzyme concentration, contact time and pH value) on the properties of the biocatalyst was determined. Then, the immobilized enzyme characterization was performed and kinetic parameters were obtained. Thermal and operational stabilities were improved compared with free commercial laccase showing its potential for continuous applications. Finally, the performance of immobilized laccase for the continuous degradation of various reactive textile dyes and of a mixture of them in batch reactors was evaluated. Two phenomena were observed: decolourization of the solutions due to dyes adsorption on the support and due to the enzyme action. A high decolourization percentage of practically all dyes in the first two cycles and an effective decolourization of the dye mixture were obtained, showing the suitability of the immobilized commercial laccase for continuous colour removal from textile industrial effluents.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7