Evans Blue is an inhibitor of nuclear factor-kappa B (NF-kappa B)-DNA binding

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Sharma, RK (Author) Other; Otsuka, M (Author) Other; Pande, V (Author) Other; Inoue, J (Author) Other; Ramos, MJ (Author) FCUP

Journal

Title: Bioorganic and Medicinal Chemistry Letters

Vol. 14

Pages: 6123-6127

ISSN: 0960-894X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 16 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-79X

DOI: 10.1016/j.bmcl.2004.07.096

Abstract (EN): Nuclear factor-kappa B (NF-KB) is an important transcription factor, involved in many immune and inflammatory responses. It is critical in HIV gene expression as it has kappa B binding sites in the HIV-1 long-terminal repeat. Hence, targeting NF-KB to prevent its DNA binding holds a significant therapeutic potential. In this context, we report Evans Blue as a novel inhibitor of NF-kappaB-DNA binding. Evans Blue was found to be inhibiting DNA binding of NF-KB at a low concentration of 100 PM. Further, molecular modeling studies using docking and generation of electrostatic potential maps predicted a possible binding mode of EB to the DNA binding region of NF-kappaB, consistent with the experimental activity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5