Validation of a micromethod for quantification of lutein and beta-carotene in olive oil

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Lúcia Maia (Author) Other; Susana Casal (Author) FFUP; M. Beatriz Oliveira (Author) FFUP; Paula Oliveira (Author) FEUP

Journal

Title: Journal of Liquid Chromatography and Related Technologies

Vol. 31 No. 5

Pages: 733-742

ISSN: 1082-6076

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 7 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-3WA

DOI: 10.1080/10826070701854139

Abstract (EN): A micromethod was developed and validated for the quantification of lutein and beta-carotene in olive oils. The compounds are liquid-liquid extracted with n-hexane and n,n-dimethylformamide (DMF), by vortex mixing and centrifugation. The n-hexane phase retains beta-carotene, whereas the DMF phase retains lutein. This last fraction is concentrated and analyzed by RP-HPLC/diode-array, with a gradient of acetonitrile, water, and ethyl acetate, using beta-apo-8'-carotenal as I.S. beta-carotene is quantified by UV/Vis measurements at 454nm, by the external standard method. The method shows appropriate inter- and intra-day reproducibility, accuracy, and linearity for both compounds, allowing fast analysis of several samples simultaneously, with a relatively small consumption of organic solvents. The analytical methodology was applied to several samples of commercial Portuguese PDO olive oils.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10