Molinate biodegradation in soils: natural attenuation versus bioaugmentation

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Ana Rita Lopes (Author) FEUP; Anthony S. Danko (Author) FEUP; Célia M. Manaia (Author) Other; Olga C. Nunes (Author) FEUP

Journal

Title: Applied Microbiology and Biotechnology

Vol. 97 No. 6

Pages: 2691-2700

ISSN: 0175-7598

Publisher: Springer Nature

Indexing

ISI Proceedings

ISI Web of Knowledge - 10 Citations

Scopus - 15 Citations

Scientific classification

CORDIS: Natural sciences

FOS: Natural sciences > Biological sciences

Associated Projects

POCI/AMB/59836/2004 - Biorremediação de solos e águas contaminadas com pesticidas

PTDC/AMB/66653/2006 - Caracterização de uma nova via metabólica: mineralização do herbicida molinato

UI0511 - PROJECTO ESTRATÉGICO - UI 511 - 2013-2014

Other information

Authenticus ID: P-002-020

DOI: 10.1007/s00253-012-4096-y

Abstract (EN): The aims of the present study were to assess the potential of natural attenuation or bioaugmentation to reduce soil molinate contamination in paddy field soils and the impact of these bioremediation strategies on the composition of soil indigenous microbiota. A molinate mineralizing culture (mixed culture DC) was used as inoculum in the bioaugmentation assays. Significantly higher removal of molinate was observed in bioaugmentation than in natural attenuation microcosms (63 and 39 %, respectively) after 42 days of incubation at 22 A degrees C. In the bioaugmentation assays, the impact of Gulosibacter molinativorax ON4(T) on molinate depletion was observed since the gene encoding the enzyme responsible for the initial molinate breakdown (harboured by that actinobacterium) was only detected in inoculated microcosms. Nevertheless, the exogenous mixed culture DC did not overgrow as the heterotrophic counts of the bioaugmentation microcosms were not significantly different from those of natural attenuation and controls. Moreover, the actinobacterial clone libraries generated from the bioaugmentation microcosms did not include any 16S rRNA gene sequences with significant similarity to that of G. molinativorax ON4(T). The multivariate analysis of the 16S rRNA DGGE patterns of the soil microcosm suggested that the activity of mixed culture DC did not affect the soil bacterial community structure since the DGGE patterns of the bioaugmentation microcosms clustered with those of natural attenuation and controls. Although both bioremediation approaches removed molinate without indigenous microbiota perturbation, the results suggested that bioaugmentation with mixed culture DC was more effective to treat soils contaminated with molinate.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10