Salsipaludibacter albus gen. nov., sp. nov., a novel actinobacterial strain isolate from a Portuguese solar saltern and proposal of Salsipaludibacteraceae fam. nov. and Salsipaludibacterales ord. nov.

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Almeida, E (Author) Other; Henriques, V (Author) Other; Wiegand, S (Author) Other; Albuquerque, L (Author) Other; Schumann, P (Author) Other; Kohn, T (Author) Other; Jogler, C (Author) Other; da Costa, MS (Author) Other; Lobo-da-Cunha, A (Author) Other; Carvalho, MD (Author) Other; lage, om (Author) FCUP

Journal

Title: International Journal of Systematic and Evolutionary Microbiology

Vol. 72

ISSN: 1466-5026

Publisher: Microbiology Society

Indexing

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Other information

Authenticus ID: P-00W-448

DOI: 10.1099/ijsem.0.005228

Abstract (EN): A novel micro-organism designated AS10(T) was isolated from dry salt collected from Aveiro saltern in the north of Portugal. Cells were Gram-stain-positive, non-motile, non-endospore-forming, rod-shaped and aerobic. Strain AS10(T) was characterized by long filaments of rod-shaped cells, presenting also coccoid cellular forms at the end of the filaments, unveiling some pleomorphism. Rod-shaped cells varied from 0.3 to 0.6 mu m wide and from 0.6 to 2 mu m long. Growth of AS10(T) occurred at 15-40 degrees C (optimum, 20-30 degrees C), 0-10% (w/v) NaCl (optimum, 2%) and pH 4.5-11.0 (optimum, pH 8.0-11.0). The peptidoglycan type was A1 Upsilon-type with 3-hydroxy-diaminopimelic acid. The major fatty acids were C-16(:0), iso-C-1(4:)0, C-17(:0) and C-1(4:)0. The major respiratory quinone was MK-9(H-4). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AS10(T) was similar to actinobacterial members of the class Nitriliruptoria. with Nitriliruptor alkaliphilus ANL-isor being the closest relative the species with a sequence pairwise similarity of 91.21%. Average nucleotide identity, average amino acid identity and in silico DNA-DNA hybridization values between strain AS10(T) and N. alkaliphilus ANL-iso2(T) were 71.34, 53.57 and 18.90%, respectively. The genome DNA G+C content of AS10(T) was 71.8 mol%. Based on genomic, phylogenetic, phenotypic and chemotaxonomic studies, we describe a new species of a novel genus represented by strain AS10(T) (=LMG 31937(T)=CECT 30148(T)) for which we propose the name Salsipaludibacter albus gen. nov., sp. nov. We also propose that this organism represents a new family named Salsipaludibacteraceae fam. nov. of a novel order named Salsipaludibacterales ord. nov.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14