Release profile and transscleral permeation of triamcinolone acetonide loaded nanostructured lipid carriers (TA-NLC): in vitro and ex vivo studies

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Araujo, J (Author) Other; Garcia, ML (Author) Other; Mallandrich, M (Author) Other; Souto, EB (Author) Other; Calpena, AC (Author) Other

Journal

Title: Nanomedicine: Nanotechnology, Biology, and Medicine

Vol. 8

Pages: 1034-1041

ISSN: 1549-9634

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 50 Citations

Other information

Authenticus ID: P-002-7JW

DOI: 10.1016/j.nano.2011.10.015

Abstract (EN): Nanostructured lipid carriers (NLC) have been developed for sustained release of triamcinolone acetonide (TA), a corticosteroid commonly indicated for macular edema, neovascularization, and other ocular inflammatory disorders. TA-NLC were prepared by high-pressure homogenization and characterized for in vitro release by dialysis bag. Ex vivo permeation profile was assessed using rabbit sclera isolated and mounted in Franz diffusion cells. TA-NLC were placed in episcleral donor compartment and choroidal side was perfused with HEPES buffer. Tissue sections underwent drug wash-out, following analysis by validated RP-HPLC of drug content and perfused fractions collected over 24 hours. Drug release followed one-order kinetics and permeability studies confirmed that TA is able to diffuse across rabbit sclera in sustained profile, following zero-order kinetics. Strong tissue binding was observed, providing a drug depot. These findings are of potential use when designing future TA therapy strategies for ocular diseases of posterior segment. From the Clinical Editor: In this study of triamcinolone acetonide loaded nanosize lipid carriers, potential ophthalmological applications are investigated including its trans-scleral permeation and release profile. Future in vitro studies are anticipated to establish this novel lipid carrier as a future therapeutic option for ocular diseases of the posterior segment.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8