New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Nilza Ribeiro (Author) Other; Aureliana Sousa (Author) Other; Cassilda Cunha Reis (Author) Other; Ana Leite Oliveira (Author) Other; Pedro L. Granja (Author) Other; Fernando Mendes Monteiro (Author) FEUP; Susana R. Sousa (Author) Other

Journal

Title: Nanomedicine: Nanotechnology, Biology, and Medicine

Vol. 33

Pages: 1-13

ISSN: 1549-9634

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 0 Citations

Other information

Authenticus ID: P-00T-E3B

DOI: 10.1016/j.nano.2020.102353

Abstract (EN): This study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13