SUBTYPING OF ALKYLATED HUMAN OROSOMUCOID - EVIDENCE FOR A DUPLICATED GENE, ORM1-ASTERISK-F2S

• Tipo: Artigo em Revista Científica Internacional
• Data: 1993

Autores

ROCHA, J (Autor) FCUP; AMORIM, A (Autor) FCUP; LUCKENBACH, C (Autor) Outra; KOMPF, J (Autor) Outra; RITTER, H (Autor) Outra

Revista

Título: Electrophoresis

Vol. 14

Páginas: 235-237

ISSN: 0173-0835

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 6 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-001-N9V

DOI: 10.1002/elps.1150140138

Abstract (EN): Isoelectric focusing of human orosomucoid (ORM) was studied following different sample treatment. It is shown that: (i) alkylation with iodoacetamide leads to a drastic change in the isoelectric point (pI) of both ORM1 F2 and ORM2 A gene products and greatly improves the discrimination between ORM1 F1 and ORM1 F2; (ii) previous reduction of the molecule with dithiothreitol partially inhibits the pI transitions with resultant artifactual ORM1 F1F2S patterns that correspond in most cases to F2S phenotypes. With the technique now described, the persistence of three ORM1 gene products was found in only one individual and the segregation analysis is consistent with the existence of a rare ORM1*F2S haplotype.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 3