Development and validation of a fast procedure to analyze amoxicillin in river waters by direct-injection LC-MS/MS

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Vera Homem (Autor) FEUP; Arminda Alves (Autor) FEUP; Lúcia Santos (Autor) FEUP

Revista

Título: Journal of Chemical Education

Vol. 91 Nº 11

Páginas: 1961-1965

ISSN: 0021-9584

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 9 Citações

Scopus - 14 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia do ambiente

Projetos Associados

UI0511 - PROJECTO ESTRATÉGICO - UI 511 - 2013-2014

LEPAE/CEFT - RL2 - (Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health

Outras Informações

ID Authenticus: P-00A-10N

DOI: 10.1021/ed500482j

Abstract (EN): A laboratory application with a strong component in analytical chemistry was designed for undergraduate students, in order to introduce a current problem in the environmental science field, the water contamination by antibiotics. Therefore, a simple and rapid method based on direct injection and high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and optimized for the determination of amoxicillin in river water. Students learned the main optimization steps for the improvement of the LC-MS/MS methodology and the correct procedure to validate an analytical method (draw a calibration curve, determine detection and quantification limits, precision, and accuracy). This laboratory experiment was successfully applied by students and enables the analysis of a large number of samples in a short period of time because of the short run time (3 min).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5