Alkaline density gradient floatation of membranes: Polypeptide composition of the mammalian peroxisomal membrane

• Tipo: Artigo em Revista Científica Internacional
• Data: 1999

Autores

gouveia, amm (Autor) Outra; reguenga, c (Autor) Outra; oliveira, mem (Autor) Outra; eckerskorn, c (Autor) Outra; sa-miranda, c (Autor) Outra; azevedo, je (Autor) ICBAS

Revista

Título: Analytical Biochemistry

Vol. 274

Páginas: 270-277

ISSN: 0003-2697

Editora: Elsevier

Indexação

ISI Web of Knowledge - 17 Citações

Scopus - 19 Citações

Outras Informações

ID Authenticus: P-001-32S

DOI: 10.1006/abio.1999.4281

Abstract (EN): A method for purification of the peroxisomal membrane from rat liver is described. The procedure consists of floating the (contaminated) peroxisomal membranes through an alkaline sucrose density gradient. A good resolution between the peroxisomal membrane and other membrane systems is achieved. Using these floated peroxisomal membranes we have determined that only 7.8 +/- 0.9% of the total peroxisomal protein is alkali resistant. The polypeptide composition of these highly pure peroxisomal membranes was analyzed by SDS-PAGE. Bands corresponding to polypeptides with apparent molecular masses of 15, 18, 22, 24, 26, 29, 35, 36, 38, 40, 52, 55, 70, 74-77, and 88 kDa are detected upon Coomassie blue staining of polyacrylamide gels. The identity of several of these polypeptides was determined by N-terminal sequencing and Western blotting analysis. (C) 1999 Academic Press.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8