Interleukin-6 regulates the phenotype of the immune response to a tuberculosis subunit vaccine

• Tipo: Artigo em Revista Científica Internacional
• Data: 2001

Autores

Leal, IS (Autor) Outra; Florido, M (Autor) Outra; Andersen, P (Autor) Outra; Appelberg, R (Autor) ICBAS

Revista

Título: Immunology

Vol. 103

Páginas: 375-381

ISSN: 0019-2805

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 13 Citações

Scopus - 14 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

CORDIS: Ciências da Saúde > Ciências Médicas > Medicina > Infecções

Outras Informações

ID Authenticus: P-000-V2H

DOI: 10.1046/j.1365-2567.2001.01244.x

Abstract (EN): We investigated the role of interleukin-6 (IL-6) in the development of the immune response to a subunit vaccine against tuberculosis consisting of the culture filtrate proteins of Mycobacterium tuberculosis emulsified in the adjuvant dimethyldioctadecylammonium bromide (DDA). C57Bl/6 mice immunized with this vaccine developed a strong T helper 1 (Th1) response characterized by an increased production of interferon-gamma (IFN-gamma) secreted by CD4(+) T cells. Neutralization of IL-6 during in vivo priming resulted in marked reduction in the ability of T cells to secrete IFN-gamma and IL-2 and to proliferate. IL-6 gene-disrupted mice primed with the vaccine showed a decrease in the number of IFN-gamma -producing cells and an increase in IL-4-secreting cells as compared to control mice. In contrast, neutralization of IL-6 during a boost of the vaccine in previously primed mice did not affect the development of IFN-gamma -producing cells but still increased the number of IL-4-producing cells. Our work shows that IL-6 plays a major role in the priming but not in the later expression of a Th1 response to a tuberculosis vaccine.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7