Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Lucia H M L M Santos (Autor) Outra; Araujo, AN (Autor) FFUP; Boaventura Reis (Autor) Outra; Montenegro, MCBSM (Autor) FFUP

Revista

Título: Journal of Automated Methods and Management in Chemistry

Vol. 2010

Páginas: 1-7

ISSN: 1463-9246

Editora: HINDAWI PUBLISHING CORPORATION

Indexação

ISI Web of Knowledge - 2 Citações

ISI Web of Science

Scopus - 3 Citações

Pubmed / Medline

Classificação Científica

CORDIS: Ciências da Saúde > Ciências farmacológicas > Farmácia

FOS: Ciências da engenharia e tecnologias > Outras ciências da engenharia e tecnologias

Outras Informações

ID Authenticus: P-003-BJ6

DOI: 10.1155/2010/913207

Resumo (PT): A new flow procedure based on multicommutation with chemiluminometric detection was developed to quantify gentamicin sulphate in pharmaceutical formulations. This approach is based on gentamicin's ability to inhibit the chemiluminometric reaction between luminol and hypochlorite in alkaline medium, causing a decrease in the analytical signal. The inhibition of the analytical signal is proportional to the concentration of gentamicin sulphate, within a linear range of 1 to 4 μg mL-1 with a coefficient variation <3%. A sample throughput of 55 samples h-1 was obtained. The developed method is sensitive, simple, with low reagent consumption, reproducible, and inexpensive, and when applied to the analysis of pharmaceutical formulations (eye drops and injections) it gave results with RSD between 1.10 and 4.40%. <br> <br> <a target="_blank" href="http://www.hindawi.com/journals/jammc/2010/913207/ "> Texto integral </a> <br> <br>

Abstract (EN): A new flow procedure based on multicommutation with chemiluminometric detection was developed to quantify gentamicin sulphate in pharmaceutical formulations. This approach is based on gentamicin's ability to inhibit the chemiluminometric reaction between luminol and hypochlorite in alkaline medium, causing a decrease in the analytical signal. The inhibition of the analytical signal is proportional to the concentration of gentamicin sulphate, within a linear range of 1 to 4 mu g mL(-1) with a coefficient variation <3%. A sample throughput of 55 samples h(-1) was obtained. The developed method is sensitive, simple, with low reagent consumption, reproducible, and inexpensive, and when applied to the analysis of pharmaceutical formulations (eye drops and injections) it gave results with RSD between 1.10 and 4.40%.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7