In Vivo Imaging of Glycol Chitosan-Based Nanogel Biodistribution

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Pereira, P (Autor) Outra; Alexandra Correia (Autor) ICBAS; Gama, FM (Autor) Outra

Revista

Título: Macromolecular Bioscience

Vol. 16

Páginas: 432-440

ISSN: 1616-5187

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 16 Citações

Outras Informações

ID Authenticus: P-00K-9B1

DOI: 10.1002/mabi.201500267

Abstract (EN): The preclinical development of nanomedicines raises several challenges and requires a comprehensive characterization. Among them is the evaluation of the biodistribution following systemic administration. In previous work, the biocompatibility and in vitro targeting ability of a glycol chitosan (GC) based nanogel have been validated. In the present study, its biodistribution in the mice is assessed, using near-infrared (NIR) fluorescence imaging as a tool to track the nanogel over time, after intravenous administration. Rapid whole body biodistribution of both Cy5.5 labeled GC nanogel and free polymer is found at early times. It remains widespreadly distributed in the body at least up to 6 h postinjection and its concentration then decreases drastically after 24 h. Nanogel blood circulation half-life lies around 2 h with the free linear GC polymer presenting lower blood clearance rate. After 24 h, the blood NIR fluorescence intensity associated with both samples decreases to insignificant values. NIR imaging of the organs shows that the nanogel had a body clearance time of approximate to 48 h, because at this time point a weak signal of NIR fluorescence is observed only in the kidneys. Hereupon it can be concluded that the engineered GC nanogel has a fairly long blood circulation time, suitable for biomedical applications, namely, drug delivery, simultaneously allowing efficient and quick body clearance. [GRAPHICS] .

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9