Preanalytical stability of plasma/serum brain-derived tau

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Gonzalez-Ortiz, F (Autor) Outra; Dias, A (Autor) Outra; Turton, M (Autor) Outra; Magalhaes, R (Autor) ICBAS; Kac, PR (Autor) Outra; Correia, M (Autor) Outra; Harrison, P (Autor) Outra; Zetterberg, H (Autor) Outra; Maia, LF (Autor) Outra; Blennow, K (Autor) Outra; Karikari, TK (Autor) Outra

Revista

Título: Alzheimers & Dementia

ISSN: 1552-5260

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 12 Citações

Outras Informações

ID Authenticus: P-00Y-DZ8

DOI: 10.1002/alz.13156

Abstract (EN): INTRODUCTIONWe investigated the effects of matrix type and reagent batch changes on diagnostic performances and longitudinal trajectories of brain-derived tau (BD-tau). METHODSWe evaluated (i) Cohort 1: paired EDTA plasma and serum from Alzheimer biomarker-positive older adults versus controls (n = 26); and (ii) Cohort 2: n = 79 acute ischemic stroke patients with 265 longitudinal samples across four time points. RESULTSIn Cohort 1, plasma and serum BD-tau were strongly correlated (rho = 0.96, p < 0.0001) with similar diagnostic performances (AUCs >99%) and correlations with CSF total-tau (rho = 0.93-0.94, p < 0.0001). However, absolute concentrations were similar to 40% higher in plasma versus serum. In Cohort 2, first and repeated BD-tau measurements showed a near-perfect correlation (rho = 0.96, p < 0.0001), with no significant between-batch concentration differences. In longitudinal analyses, substituting similar to 10% of the first-run concentrations for the remeasured values showed overlapping estimated trajectories without significant differences at any time point. DISCUSSIONBD-tau has equivalent diagnostic accuracies, but non-interchangeable absolute concentrations, in plasma versus serum. Furthermore, the analytical robustness is unaffected by batch-to-batch reagent variations. HighlightsBrain-derived tau (BD-tau) is a novel blood-based biomarker that quantifies tau protein of CNS origin.Effects of preanalytical handling procedures on the quality and reproducibility of BD-tau measures are unknown.In two cohorts of n = 105 participants, we compared BD-tau concentrations and diagnostic performances in paired plasma and serum samples, and evaluated impacts of batch-to-batch reagent variations.Paired plasma and serum showed equivalent diagnostic performances to separate amyloid-positive AD from amyloid-negative controls, indicating both can be used independently.Repeated measurements and longitudinal trajectories of plasma BD-tau were unaffected by batch-to-batch reagent variation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7