Proteomics of industrial fungi: trends and insights for biotechnology

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2011

Autores

de Oliveira, JMPF (Autor) Outra; de Graaff, LH (Autor) Outra

Revista

Título: Applied Microbiology and Biotechnology

Vol. 89

Páginas: 225-237

ISSN: 0175-7598

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 37 Citações

Scopus - 42 Citações

Outras Informações

ID Authenticus: P-00F-WSG

DOI: 10.1007/s00253-010-2900-0

Abstract (EN): Filamentous fungi are widely known for their industrial applications, namely, the production of food-processing enzymes and metabolites such as antibiotics and organic acids. In the past decade, the full genome sequencing of filamentous fungi increased the potential to predict encoded proteins enormously, namely, hydrolytic enzymes or proteins involved in the biosynthesis of metabolites of interest. The integration of genome sequence information with possible phenotypes requires, however, the knowledge of all the proteins in the cell in a system-wise manner, given by proteomics. This review summarises the progress of proteomics and its importance for the study of biotechnological processes in filamentous fungi. A major step forward in proteomics was to couple protein separation with high-resolution mass spectrometry, allowing accurate protein quantification. Despite the fact that most fungal proteomic studies have been focused on proteins from mycelial extracts, many proteins are related to processes which are compartmentalised in the fungal cell, e.g. beta-lactam antibiotic production in the microbody. For the study of such processes, a targeted approach is required, e.g. by organelle proteomics. Typical workflows for sample preparation in fungal organelle proteomics are discussed, including homogenisation and sub-cellular fractionation. Finally, examples are presented of fungal organelle proteomic studies, which have enlarged the knowledge on areas of interest to biotechnology, such as protein secretion, energy production or antibiotic biosynthesis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13