Flexible and expeditious assay for quantitative monitoring of alpha-amylase and amyloglucosidase activities

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Oliveira, HM (Autor) Outra; Pinheiro, AQ (Autor) Outra; Antonio J M Fonseca (Autor) ICBAS; A. Cabrita (Autor) ICBAS; Maia, MRG (Autor) ICBAS

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: METHODSX

Vol. 6

Páginas: 246-258

ISSN: 2215-0161

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 6 Citações

Outras Informações

ID Authenticus: P-00Q-E8D

DOI: 10.1016/j.mex.2019.01.007

Abstract (EN): The monitoring of the activity of alpha-amylase and amyloglucosidase is an important tool for studying their role in the hydrolysis of starch. Here we introduced an improved method capable to measure the activity of alpha-amylase and amyloglucosidase from different sources based on a quantitative starch-iodine assay. The developments of the assay sought the consistent preparation of the reagents, the rescale of the assay and the adjustment of the sensitivity. This was complemented by a glucose yield assay for amyloglucosidase that allowed a secondary source of information when insoluble starches were studied. The proposed method showed high precision in long-term use (RSD <6.3%). Furthermore, all experimental conditions can be adapted according to the equipment available at each laboratory, transforming this method in a broadband analytical tool for screening alpha-amylase and amyloglucosidase activities. Tailorable assay based on the starch-iodine staining for the determination of alpha-amylase and amyloglucosidase activities. Enhanced consistence of reagent preparation. High intra-day and inter-day reproducibility. (C) 2019 The Authors. Published by Elsevier B.V.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13