Acto-myosin force organization modulates centriole separation and PLK4 recruitment to ensure centriole fidelity

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Vitiello, E (Autor) Outra; Moreau, P (Autor) Outra; Nunes, V (Autor) Outra; Mettouchi, A (Autor) Outra; Maiato, H (Autor) Outra; J Ferreira (Autor) FMUP; Wang, I (Autor) Outra; Balland, M (Autor) Outra

Revista

Título: Nature Communications

Vol. 10

ISSN: 2041-1723

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 9 Citações

Scopus - 11 Citações

Outras Informações

ID Authenticus: P-00Q-303

DOI: 10.1038/s41467-018-07965-6

Abstract (EN): The presence of aberrant number of centrioles is a recognized cause of aneuploidy and hallmark of cancer. Hence, centriole duplication needs to be tightly regulated. It has been proposed that centriole separation limits centrosome duplication. The mechanism driving centriole separation is poorly understood and little is known on how this is linked to centriole duplication. Here, we propose that actin-generated forces regulate centriole separation. By imposing geometric constraints via micropatterns, we were able to prove that precise actomyosin force arrangements control direction, distance and time of centriole separation. Accordingly, inhibition of acto-myosin contractility impairs centriole separation. Alongside, we observed that organization of acto-myosin force modulates specifically the length of S-G2 phases of the cell cycle, PLK4 recruitment at the centrosome and centriole fidelity. These discoveries led us to suggest that acto-myosin forces might act in fundamental mechanisms of aneuploidy prevention.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12