Hydrolysis of butteroil by immobilized lipase using a hollow-fiber reactor: Part II. Uniresponse kinetic studies

• Tipo: Artigo em Revista Científica Internacional
• Data: 1992

Autores

F. Xavier Malcata (Autor) Outra; Hill, CG (Autor) Outra; Amundson, CH (Autor) Outra

Revista

Título: Biotechnology and Bioengineering

Vol. 39

Páginas: 984-1001

ISSN: 0006-3592

Editora: Wiley-Blackwell

Indexação

Scopus - 61 Citações

Outras Informações

ID Authenticus: P-008-VHV

Abstract (EN): A lipase from Aspergillus niger immobilized by adsorption on microporous, polypropylene hollow fibers was used to effect the hydrolysis of the glycerides of melted butterfat at 40°C and pH 7.0. McIlvane buffer was pumped through the lumen and melted butterfat was pumped cocurrently through the shell side of a shell-and-tube reactor. Nonlinear regression methods were employed to determine the kinetic parameters of three nested rate expressions derived from a Ping Pong Bi Bi enzymatic mechanism coupled with three nested rate expressions for the thermal deactivation of the enzyme. For the reaction conditions used in this research, a four-parameter rate expression (which includes a two-parameter deactivation rate expression and a two-parameter hydrolysis rate expression) is sufficient to model the overall release of free fatty acids from the triglycerides of butterfat as a function of space time and time elapsed after immobilization. At a space time of 3.7 h immediately after immobilization of lipase, 50% of the fatty acid residues esterified in the sn-1,3 positions of the triglycerides can be released in the hollow-fiber reactor.A lipase from Aspergillus niger immobilized by adsorption on microporous, polypropylene hollow fibers was used to effect the hydrolysis of the glycerides of melted butterfat at 40°C and pH 7.0. McIlvane buffer was pumped through the lumen and melted butterfat was pumped concurrently through the shell side of a shell-and-tube reactor. Nonlinear regression methods were employed to determine the kinetic parameters of three nested rate expressions derived from a Ping Pong Bi Bi enzymatic mechanism coupled with three nested rate expressions for the thermal deactivation of the enzyme. For the reaction conditions used in this research, a four-parameter rate expression (which includes a two-parameter deactivation rate expression and a two-parameter hydrolysis rate expression) is sufficient to model the overall release of free fatty acids from the triglycerides of butterfat as a function of space time and time elapsed after immobilization. At a space time of 3.7 h immediately after immobilization of lipase, 50% of the fatty acid residues esterified in the sn-1,3 positions of the triglycerides can be released in the hollow-fiber reactor.

Idioma: Inglês

Tipo (Avaliação Docente): Científica