Isothermal titration calorimetry (ITC): a standard operating procedure (SOP)

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Bastos, M (Autor) FCUP; Velazquez Campoy, A (Autor) Outra

Revista

Título: European Biophysics Journal

Vol. 50

Páginas: 363-371

ISSN: 0175-7571

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 10 Citações

Outras Informações

ID Authenticus: P-00T-J8Q

DOI: 10.1007/s00249-021-01509-5

Abstract (EN): Isothermal titration calorimetry (ITC) is currently widely used in many applied areas of research, spanning protein-ligand binding, metal-ligand interactions, DNA/DNA or protein/DNA interactions, partition to membranes, and polymer surfactant interactions, to mention just a few. This is due to the availability of commercial instruments, and thus the production and spread of an accepted and widely followed SOP is felt by most users, in an effort to produce results that are scientifically correct and comparable. Therefore, within the efforts of Working Group 4 of the ARBRE-MOBIEU COST Action (CA15126), this ITC SOP was generated, alongside SOPs for several other biophysical techniques. Here, we discuss the factors that are fundamental for good experimental design and that need to be carefully considered, as well as machine calibration, in particular chemical calibration, linked to another outcome of Working Group 4 on ITC benchmarking, to be also published in this Special Issue.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9