Can nuclear magnetic resonance (NMR) spectroscopy reveal different metabolic signatures for lung tumours?

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Duarte, IF (Autor) Outra; Rocha, CM (Autor) Outra; Barros, AS (Autor) Outra; Gil, AM (Autor) Outra; Goodfellow, BJ (Autor) Outra; Carreira, IM (Autor) Outra; Bernardo, J (Autor) Outra; Gomes, A (Autor) Outra; Sousa, V (Autor) Outra; Carvalho, L (Autor) Outra

Revista

Título: Virchows Archiv

Vol. 457

Páginas: 715-725

ISSN: 0945-6317

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 32 Citações

Scopus - 32 Citações

Outras Informações

ID Authenticus: P-003-0VV

DOI: 10.1007/s00428-010-0993-6

Abstract (EN): This study aims to evaluate the potential of H-1 NMR spectroscopy, combined with multivariate statistics, for discriminating between tumour and non-involved (control) pulmonary parenchyma and for providing biochemical information on different histological types. Paired tissue samples from 24 primary lung tumours were directly analysed by high-resolution magic angle spinning (HRMAS) H-1 NMR spectroscopy (500 MHz), and their spectral profiles subjected to principal component analysis (PCA) and partial least squares regression discriminant analysis (PLS-DA). Tumour and adjacent control parenchyma were clearly discriminated in the PLS-DA model with a high level of sensitivity (95% of tumour samples correctly classified) and 100% specificity (no false positives). The metabolites giving rise to this separation were mainly lactate, glycerophosphocholine, phosphocholine, taurine, reduced glutathione and uridine di-phosphate (elevated in tumours) and glucose, phosphoethanolamine, acetate, lysine, methionine, glycine, myo- and scyllo-inositol (reduced in tumours compared to control tissues). Furthermore, PLS-DA of a sub-set of tumour samples allowed adenocarcinomas to be discriminated from carcinoid tumours and epidermoid carcinomas, highlighting differences in metabolite levels between these histological types, and therefore revealing valuable knowledge on the biochemistry of different types of bronchial-pulmonary carcinomas.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11