Pullulan microneedle patches for the efficient transdermal administration of insulin envisioning diabetes treatment

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Fonseca, DFS (Autor) Outra; Paulo Costa (Autor) FFUP; Almeida, IF (Autor) FFUP; Dias Pereira, P (Autor) Outra; Correia Sa, I (Autor) Outra; Bastos, V (Autor) Outra; Oliveira, H (Autor) Outra; Margarida Araújo (Autor) ICBAS; Morato, M (Autor) FFUP; Vilela, C (Autor) Outra; Silvestre, AJD (Autor) Outra; Freire, CSR (Autor) Outra

Revista

Título: Carbohydrate Polymers

Vol. 241

ISSN: 0144-8617

Editora: Elsevier

Indexação

ISI Web of Knowledge - 58 Citações

Scopus - 61 Citações

Outras Informações

ID Authenticus: P-00S-412

DOI: 10.1016/j.carbpol.2020.116314

Abstract (EN): The present study reports the fabrication of dissolvable microneedle (MN) patches using pullulan (PL), a watersoluble polysaccharide with excellent film-forming ability, for the transdermal administration of insulin, envisioning the non-invasive treatment of diabetes. PL MNs patches were successfully prepared by micromoulding and revealed good thermal stability (T-dmax = 294 degrees C) and mechanical properties (> 0.15 N needle(-1)), penetrating skin up to 381 mu m depth, as revealed by in vitro skin tests. After application into human abdominal skin in vitro, the MNs dissolved within 2 h releasing up to 87% of insulin. When stored at 4, 20 and 40 degrees C for 4 weeks, insulin was able to retain its secondary structure, as shown by circular dichroism spectropolarimetry. The prepared PL MNs were non-cytotoxic towards human keratinocytes, being suitable for skin application. These findings suggest that PL MNs have potential to deliver insulin transdermally, thus avoiding its subcutaneous administration.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9