Loss of functional E-cadherin renders cells more resistant to the apoptotic agent taxol in vitro

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Ferreira, P (Autor) Outra; Oliveira, MJ (Autor) Outra; Beraldi, E (Autor) Outra; Mateus, AR (Autor) Outra; Nakajima, T (Autor) Outra; Gleave, M (Autor) Outra; Yokota, J (Autor) Outra; Carneiro F (Autor) FMUP; Huntsman, D (Autor) Outra; Seruca, R (Autor) FMUP; Suriano, G (Autor) Outra

Revista

Título: Experimental Cell Research

Vol. 310

Páginas: 99-104

ISSN: 0014-4827

Editora: Elsevier

Indexação

ISI Web of Knowledge - 44 Citações

Scopus - 46 Citações

Outras Informações

ID Authenticus: P-000-0XJ

DOI: 10.1016/j.yexcr.2005.07.010

Abstract (EN): Experimental evidence supports a role for E-cadherin in suppressing invasion, metastasis, and proliferation. Germline mutations of the E-cadherin represent the genetic cause of hereditary diffuse gastric cancer (HDGC). In this type of tumor, isolated cancer cells permeate the basal membrane and paradoxically survive in the gastric wall in the absence of contact with neighbor epithelial cells or with the extracellular matrix. This suggests that upon E-cadherin deregulation, cells acquired resistance to apoptosis. To test this hypothesis, CHO cells stably expressing either wild-type E-cadherin or the HDGC-related germline mutations T340A and V832M were seeded either on a thin layer of collagen type I or on plastic and then subjected to the apoptotic agent taxol. We found that in vitro functional E-cadherin renders cells more sensitive to the effect of taxol. Our results also indicate that this effect is associated to decreased level of the antiapoptotic bcl-2 protein.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6