Microtubule binding of the Drosophila DMAP-85 protein is regulated by phosphorylation in vitro

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Cambiazo, V (Autor) Outra; Logarinho, E (Autor) Outra; Pottstock, H (Autor) Outra; Sunkel, CE (Autor) ICBAS

Revista

Título: FEBS Letters

Vol. 483

Páginas: 37-42

ISSN: 0014-5793

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 9 Citações

Outras Informações

ID Authenticus: P-000-YQS

DOI: 10.1016/s0014-5793(00)02077-9

Abstract (EN): The phosphorylation of microtubule-associated proteins (MAPs) is thought to be a key factor in the regulation of microtubule (MT) stability. Previously we isolated DMAP-85, a Drosophila MAP shown to be associated with stable MTs. In this work we show that DMAP-85 phosphorylated in cell-free early embryo extracts is released from MTs, MPM-2 antibodies recognize the phosphorylated protein. In vitro, DMAP-85 can be phosphorylated by the mitotic kinase Polo affecting its binding to MTs and creating MPM-2 epitopes on the protein. The results suggest that phosphorylation of DMAP-85 might affect its MT stabilizing activity during early mitotic cycles.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6