Properties and partial characterization of the heat-shock factor from Tetrahymena pyriformis

• Tipo: Artigo em Revista Científica Internacional
• Data: 1990

Autores

Do Carmo Avides, M (Autor) Outra; Sunkel, CE (Autor) Outra; Pedro Moradas Ferreira (Autor) ICBAS; Rodrigues Pousada, C (Autor) Outra

Revista

Título: European Journal of Biochemistry

Vol. 194

Páginas: 331-336

ISSN: 0014-2956

Editora: Blackwell

Indexação

Scopus - 11 Citações

Outras Informações

ID Authenticus: P-00F-GN8

Abstract (EN): A heat-shock-factor-binding activity was identified in Tetrahymena pyriformis whole-cell extracts and was further purified by sequential heparin-agarose and sequence-specific oligonucleotide affinity chromatography. Tetrahymena heat-shock factor (HSF) was able to bind to the heat-shock elements (HSE) both before and after thermal stress, although heat shock altered both the HSE-binding affinity and the protein · DNA-complex mobility on polyacrylamide gels. The mobility difference was significantly reduced by treatment of the proteins with phosphatase. The HSE-binding proteins, isolated by oligonucleotide-affinity chromatography, migrated on SDS/polyacrylamide gels as a closely spaced doublet to about 70 kDa. Polypeptides with similar molecular mass were recovered from preparative band-shift gels indicating that both are components of the protein · DNA complex.

Idioma: Inglês

Tipo (Avaliação Docente): Científica