Fetal-maternal interface impedance parallels local NADPH oxidase related superoxide production

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Guedes Martins, L (Autor) Outra; Silva, E (Autor) Outra; Gaio, AR (Autor) FCUP; Saraiva, J (Autor) Outra; Soares, AI (Autor) Outra; Afonso, J (Autor) Outra; Macedo, F (Autor) Outra; Almeida, H (Autor) Outra

Revista

Título: Redox Biology

Vol. 5

Páginas: 114-123

ISSN: 2213-2317

Editora: Elsevier

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 6 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-00G-3G0

DOI: 10.1016/j.redox.2015.04.007

Abstract (EN): Blood flow assessment employing Doppler techniques is a useful procedure in pregnancy evaluation, as it may predict pregnancy disorders coursing with increased uterine vascular impedance, as pre-eclampsia. While the local causes are unknown, emphasis has been put on reactive oxygen species (ROS) excessive production. As NADPH oxidase (NOX) is a ROS generator, it is hypothesized that combining Doppler assessment with NOX activity might provide useful knowledge on placental bed disorders underlying mechanisms. A prospective longitudinal study was performed in 19 normal course, singleton pregnancies. Fetal aortic isthmus (AoI) and maternal uterine arteries (UtA) pulsatility index (PI) were recorded at two time points: 20-22 and 40-41 weeks, just before elective Cesarean section. In addition, placenta and placental bed biopsies were performed immediately after fetal extraction. NOX activity was evaluated using a dihydroethidium-based fluorescence method and associations to PI values were studied with Spearman correlations. A clustering of pregnancies coursing with higher and lower PI values was shown, which correlated strongly with placental bed NOX activity, but less consistently with placental tissue. The study provides evidence favoring that placental bed NOX activity parallels UtA PI enhancement and suggests that an excess in oxidation underlies the development of pregnancy disorders coursing with enhanced UtA impedance. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10