A multiplex PCR assay for identification of the red fox (Vulpes vulpes) using the mitochondrial ribosomal RNA genes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Joana Goncalves (Autor) Outra; Clive A Marks (Autor) Outra; David Obendorf (Autor) Outra; Antonio Amorim (Autor) FCUP; Filipe Pereira (Autor) Outra

Revista

Título: Conservation Genetics Resources

Vol. 7

Páginas: 45-48

ISSN: 1877-7252

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 3 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-00A-64W

DOI: 10.1007/s12686-014-0343-0

Abstract (EN): The European red fox (Vulpes vulpes) is a highly adaptable predator indigenous to the northern hemisphere. However, in Australia the red fox is a widespread exotic predator that has contributed to the decline and extinction of several native species. Here we describe a multiplex PCR assay for the molecular identification of the red fox. The identification is achieved by the generation of a diagnostic profile combining the lengths of mitochondrial ribosomal RNA (rRNA) gene regions amplified using highly conserved PCR primers. The method was tested in DNA samples from 17 species, including in mixtures. Our results demonstrate that the red fox has a unique combination of fragment lengths determined by capillary electrophoresis that can be used for its unambiguous discrimination from common domestic and wild species.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4