Evaluation of inducible promoters on the secretion of a ZZ-proinsulin fusion protein in Escherichia coli

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Mergulhao, FJM (Autor) Outra; Monteiro, GA (Autor) Outra; Larsson, G (Autor) Outra; Bostrom, M (Autor) Outra; Farewell, A (Autor) Outra; Nystrom, T (Autor) Outra; Cabral, JMS (Autor) Outra; Taipa, MA (Autor) Outra

Revista

Título: Biotechnology and Applied Biochemistry

Vol. 38

Páginas: 87-93

ISSN: 0885-4513

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 14 Citações

Scopus - 15 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-FKZ

DOI: 10.1042/ba20030043

Abstract (EN): Four inducible promoters, uspA, uspB, IacUV5 and malK, were evaluated in the expression of the fusion protein ZZ-proinsulin by Escherichia coli. The aim was to select for their effects on the most appropriate expression system (promoter and culture medium) for secretion of ZZ-proinsulin to the periplasmic space and culture medium. All the expression vectors contained the RNase III cleavage site to ensure that the mRNA translation rate remained independent of 5'-untranslated regions thus making promoter strength comparisons more accurate. The highest ZZ-proinsulin secretion yields were 6.2 mg/g of dry cell weight in the periplasmic space and 2.6 mg/g of dry cell weight in the culture medium using the malK promoter. It was also demonstrated that the use of M9 minimal medium favours secretion.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7