Microbial degradation of 17-estradiol and 17-ethinylestradiol followed by a validated HPLC-DAD method

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Ribeiro, AR (Autor) Outra; Maria F Carvalho (Autor) Outra; Carlos M M Afonso (Autor) FFUP; M E Tiritan (Autor) FFUP; Paula M L Castro (Autor) Outra

Revista

Título: Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

Vol. 45

Páginas: 265-273

ISSN: 0360-1234

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 20 Citações

Scopus - 21 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Ciências da saúde

Outras Informações

ID Authenticus: P-003-BMD

DOI: 10.1080/03601231003704523

Abstract (EN): This work aimed at studying the biodegradation of two estrogens, 17 -estradiol (E2) and 17 -ethinylestradiol (EE2), and their potential metabolism to estrone (E1) by microbial consortia. The biodegradation studies were followed by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) using a specifically developed and validated method. Biodegradation studies of the estrogens (E2 and EE2) were carried out with activated sludge (consortium A, CA) obtained from a Wastewater Treatment Plant (WWTP) and with a microbial consortium able to degrade recalcitrant compounds, namely fluorobenzene (consortium B, CB). E2 was more extensively degraded than EE2 by CA whereas CB was only able to degrade E2. The addition of acetate as a supplementary carbon source led to a faster biodegradation of E2 and EE2. E1 was detected as a metabolite only during the degradation of E2. The 16S rRNA gene sequence analyses of strains recovered from the degrading cultures revealed the presence of the genera Pseudomonas, Chryseobacterium and Alcaligenes. The genera Pseudomonas and Chryseobacterium were retrieved from cultures supplied with E2 and EE2, while the genus Alcaligenes was found in the presence of E2, suggesting that they might be involved in the degradation of these compounds.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9