A stereological study on the nuclear volume of cerebellar granule cells in aging rats

• Tipo: Artigo em Revista Científica Internacional
• Data: 1997

Autores

henrique, rmf (Autor) Outra; monteiro, raf (Autor) Outra; rocha, e (Autor) ICBAS; mariniabreu, mm (Autor) Outra

Revista

Título: Neurobiology of Aging

Vol. 18

Páginas: 199-203

ISSN: 0197-4580

Editora: Elsevier

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 19 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-001-BXA

DOI: 10.1016/s0197-4580(97)00006-7

Abstract (EN): A morphometric study an age changes of rat cerebellar cortex (Cnrs I and Grits II) was carried out. The nuclear volume of the granule cells was estimated by means of two unbiased stereological methods: the nucleator, which enabled the estimation of the number-weighted mean Volume ((V) over bar(N)), and the point-sampled intercepts method, allowing the determination of the volume-weighted mean volume ((V) over bar V-N). These parameters enabled the estimation of the coefficient of variation of nuclear volume in the number-weighted distribution [CV(V-N)]. All parameters were determined in eight age groups (2, 6, 9, 12, 15, 18, 21, and 24 months-five animals per age) and the mean Values of each age group were compared using one-way ANOVA with post hoc comparisons, and regression analysis. It was demonstrated for the first time, using unbiased techniques, that the nucleus of granule cells of the cerebellar cortex showed a significant decrease in its mean Volumes ((V) over bar(V)) with age, especially from 21 months onwards. Although CV(V-N) was not correlated with age, it was also demonstrated that these cells bear a considerable nuclear size pleomorphy that was maintained throughout the age spectrum. (C) 1997 Elsevier Science Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5