Experimental and Modeling Analysis of Synechocystis sp PCC 6803 Growth

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Miguel Lopo (Autor) Outra; Arnau Montagud (Autor) Outra; Emilio Navarro (Autor) Outra; Isabel Cunha (Autor) Outra; Andrea Zille (Autor) Outra; Pedro F Fernandez de Cordoba (Autor) Outra; Pedro Moradas Ferreira (Autor) ICBAS; Paula Tamagnini (Autor) FEUP; Javier F Urchueguia (Autor) Outra

Revista

Título: Journal of Molecular Microbiology and Biotechnology

Vol. 22

Páginas: 71-82

ISSN: 1464-1801

Editora: S. Karger AG

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 18 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-G1X

DOI: 10.1159/000336850

Abstract (EN): Background/Aims: The influence of different parameters such as temperature, irradiance, nitrate concentration, pH, and an external carbon source on Synechocystis PCC 6803 growth was evaluated. Methods: 4.5-ml cuvettes containing 2 ml of culture, a high-throughput system equivalent to batch cultures, were used with gas exchange ensured by the use of a Parafilm (TM) cover. The effect of the different variables on maximum growth was assessed by a multi-way statistical analysis. Results: Temperature and pH were identified as the key factors. It was observed that Synechocystis cells have a strong influence on the external pH. The optimal growth temperature was 33 degrees C while light-saturating conditions were reached at 40 mu E.m(-2.)s(-1). Conclusion: It was demonstrated that Synechocystis exhibits a marked difference in behavior between autotrophic and glucose-based mixotrophic conditions, and that nitrate concentrations did not have a significant influence, probably due to endogenous nitrogen reserves. Furthermore, a dynamic metabolic model of Synechocystis photosynthesis was developed to gain insights on the underlying mechanism enabling this cyanobacterium to control the levels of external pH. The model showed a coupled effect between the increase of the pH and ATP production which in turn allows a higher carbon fixation rate. Copyright (C) 2012 S. Karger AG, Basel

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12