Induction of somatic embryogenesis in Iris hollandica Hort. cv. 'Bronze Queen'

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Fidalgo, F (Autor) FCUP; Santos, A (Autor) FCUP; Oliveira, N (Autor) Outra; Santos, I (Autor) Outra; Salema, R (Autor) Outra

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY

Vol. 80

Páginas: 135-138

ISSN: 1462-0316

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 11 Citações

Classificação Científica

FOS: Ciências agrárias > Agricultura, silvicultura e pescas

Outras Informações

ID Authenticus: P-000-62G

Abstract (EN): Twin-scale explants of Iris hollandica cv. 'Bronze Queen' produced callus when cultured on a modified Murashige and Skoog medium supplemented with 9.0, 18.1, 27.2 or 36.2 muM 2,4-dichlorophenoxyacetic acid (2,4-D). Induction of embryogenic callus was obtained at 9.0 or 18.1 muM 2,4-D, while higher concentrations of the auxin (27.2 or 36.2 muM) were not effective. Embryo-like structures developed on the surface of embryogenic calli. Somatic embryo maturation was achieved when the bipolar structures produced were cultured on medium without auxin. To improve embryo germination, the developing embryos were transferred to medium supplemented with 0.95 muM abscisic acid (ABA). In this medium, mature embryos developed into normal plantlets. Histological studies confirmed that the structures exhibiting bipolar differentiation were somatic embryos. Regeneration of I. hollandica cv. 'Bronze Queen' plants through somatic embryogenesis was thus established.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4