Sequence variation and mRNA expression of the TWIST1 gene in cats with mammary hyperplasia and neoplasia

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Baptista CS (Autor) ICBAS; Santos S (Autor) Outra; Laso A (Autor) Outra; Bastos E (Autor) Outra; Ávila S (Autor) Outra; Guedes-Pinto H (Autor) Outra; Gartner F (Autor) ICBAS; Gut IG (Autor) Outra; Castrillo JL (Autor) Outra; Chaves R (Autor) Outra

Revista

Título: Veterinary Journal

Vol. 191

Páginas: 203-207

ISSN: 1090-0233

Editora: Elsevier

Indexação

ISI Web of Knowledge - 9 Citações

ISI Web of Science

Scopus - 10 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências agrárias > Ciências veterinárias

Outras Informações

ID Authenticus: P-002-D8M

DOI: 10.1016/j.tvjl.2011.01.011

Abstract (EN): In humans, a germline mutation (c.309C > G) in the TWIST1 oncogene may predispose to breast cancer and its expression has been associated with tumour progression and metastasis. In this study, the feline TWIST1 gene was screened for sequence variations in 37 neoplastic and eight hyperplastic mammary gland lesions from cats. In addition, mRNA levels were examined in 15 mammary tumours and three cases of mammary hyperplasia by quantitative real-time reverse-transcriptase PCR. Feline mammary carcinomas had significantly lower levels of expression of TWIST1 mRNA than benign mammary tumours. No variations were identified in the TWIST1 coding region in feline mammary tumours and the mutation described in humans was not detected. However, two germline variants in the TWIST1 gene intron were identified in four and three carcinomas, respectively: GQ167299:g.535delG and GQ167299:g.460C > T. There was no association between these sequence alterations and TWIST1 mRNA levels.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5