A generic crystallization-like model that describes the kinetics of amyloid fibril formation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Rosa Crespo (Autor) FEUP; Fernando A Rocha (Autor) FEUP; Ana M Damas (Autor) ICBAS; Pedro M Martins (Autor) ICBAS

Revista

Título: Journal of Biological Chemistry

Vol. 287 Nº 36

Páginas: 30585-30594

ISSN: 0021-9258

Editora: Elsevier

Indexação

ISI Web of Knowledge - 46 Citações

ISI Web of Science

Scopus - 58 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia química

Projetos Associados

PTDC/BIA-PRO/101260/2008 - Crescimento de Cristais de Proteínas com Propriedades de Difracção de Raios-X de Elevada Qualidade

Outras Informações

ID Authenticus: P-002-6SX

DOI: 10.1074/jbc.m112.375345

Abstract (EN): Associated with neurodegenerative disorders such as Alzheimer, Parkinson, or prion diseases, the conversion of soluble proteins into amyloid fibrils remains poorly understood. Extensive "in vitro" measurements of protein aggregation kinetics have been reported, but no consensus mechanism has emerged until now. This contribution aims at overcoming this gap by proposing a theoretically consistent crystallization-like model (CLM) that is able to describe the classic types of amyloid fibrillization kinetics identified in our literature survey. Amyloid conversion represented as a function of time is shown to follow different curve shapes, ranging from sigmoidal to hyperbolic, according to the relative importance of the nucleation and growth steps. Using the CLM, apparently unrelated data are deconvoluted into generic mechanistic information integrating the combined influence of seeding, nucleation, growth, and fibril breakage events. It is notable that this complex assembly of interdependent events is ultimately reduced to a mathematically simple model, whose two parameters can be determined by little more than visual inspection. The good fitting results obtained for all cases confirm the CLM as a good approximation to the generalized underlying principle governing amyloid fibrillization. A perspective is presented on possible applications of the CLM during the development of new targets for amyloid disease therapeutics.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10